Influence of an Amino Acid Composition enhanced with Cold Plasma Radiation on Psychological Stress: A Blood Test, Gas Discharge Visualisation and Biofeedback Approach

Authors

  • Raul Valverde Concordia University
  • E.A. Gavrilova North-Western Medical University
  • Churganov O.A. North-Western Medical University
  • K.G Korotkov Biowell Corporation, USA

DOI:

https://doi.org/10.38140/ijspsy.v5i1.1616

Keywords:

Emotional factors, Environmental factors, First-year university students, Social integration

Abstract

This study aimed to demonstrate the effect of enhanced amino acid compositions with cold plasma on human psychological stress by using blood tests, biofeedback, and gas discharge visualisation (GDV) techniques for stress measurements. An open, randomised, placebo-controlled trial for 30 days was conducted. 70 healthy people aged 35-65, men and women, were measured initially, randomly divided into three groups (experimental, control, and placebo), and measured 30 days later for changes in stress levels. Twenty people used amino acid composition; 30 used the same amino acid composition processed with cold plasma radiation, while 20 used a placebo. The ethics committee of the Federal State Budget Institution "Saint-Petersburg Scientific-Research Institute for Physical Culture," St. Petersburg, Russia, approved the study protocol. All participants signed an informed consent form, where a written and oral explanation of the research protocol was provided. Blood, biofeedback, and GDV test results were presented to show differences in stress levels during the experiment. After 30 days, results for experimental and control groups were presented. Amino acids processed by the radiation of a cold plasma – enhanced with Igniton particles - had the most significant effect on stress levels. The results suggested that enhanced amino acid compositions significantly affected human stress levels during the longitude period. Stress reduction in humans can significantly influence disease prevention and health maintenance, ultimately extending human life expectancy.

References

Abu Rached, N., Kley, S., Storck, M., Meyer, T., & Stücker, M. (2023). Cold Plasma Therapy in Chronic Wounds—A Multicenter, Randomized Controlled Clinical Trial (Plasma on Chronic Wounds for Epidermal Regeneration Study): Preliminary Results. Journal of Clinical Medicine, 12(15), 5121. https://doi.org/10.3390/jcm12155121

Al Osman, H., Eid, M., & El Saddik, A. (2014). U-biofeedback: a multimedia-based reference model for ubiquitous biofeedback systems. Multimedia tools and applications, 72, 3143-3168.

Chen, H. J., Hsu, L. S., Shia, Y. T., Lin, M. W., & Lin, C. M. (2012). The ?-catenin/TCF complex as a novel target of resveratrol in the Wnt/?-catenin signaling pathway. Biochemical pharmacology, 84(9), 1143-1153.

Chi, Y., & Sauve, A. A. (2013). Nicotinamide riboside, a trace nutrient in foods, is a vitamin B3 with effects on energy metabolism and neuroprotection. Current Opinion in Clinical Nutrition & Metabolic Care, 16(6), 657-661. https://doi.org/10.1097/MCO.0b013e32836510c0

Chiao, R. Y., Kozhekin, A. E., & Kurizki, G. (1996). Tachyonlike excitations in inverted two-level media. Physical review letters, 77(7), 1254.

Deepak, G. D. (2022). Review on recent advances in cold plasma technology. The European Physical Journal Applied Physics, 97, 39. https://doi.org/10.1051/epjap/2022210275

Du, M. Z., Liu, S., Zeng, Z., Alemayehu, L. A., Wei, W., & Guo, F. B. (2018). Amino acid compositions contribute to the proteins’ evolution due to their abundances and genomic GC content. Scientific Reports, 8(1), 7382. https://doi.org/10.1038/s41598-018-25364-1

Du, Y., Yang, F., Yu, H., Xie, Y., & Yao, W. (2022). Improving food drying performance by cold plasma pretreatment: A systematic review. Comprehensive Reviews in Food Science and Food Safety, 21(5), 4402-4421. https://doi.org/10.1111/1541-4337.13027

Fabjanowicz, M., P?otka-Wasylka, J., & Namie?nik, J. (2018). Detection, identification and determination of resveratrol in wine. Problems and challenges. TrAC Trends in Analytical Chemistry, 103, 21-33. https://doi.org/10.1016/j.trac.2018.03.006

Gao, G., Wu, J., Wei, Z., & Li, X. (2023). Effects of low?pressure radio?frequency cold plasma on the biochemical parameters and fatty acid profile of wheat flours. Cereal Chemistry, 100(2), 393-413. https://doi.org/10.1002/cche.10618

Gao, Y., Wang, F., Song, Y., & Liu, H. (2020). The status of and trends in the pharmacology of berberine: a bibliometric review [1985–2018]. Chinese medicine, 15, 1-13. https://doi.org/10.1186/s13020-020-0288-z

Georgiou, P., Zanos, P., Bhat, S., Tracy, J. K., Merchenthaler, I. J., McCarthy, M. M., & Gould, T. D. (2018). Dopamine and stress system modulation of sex differences in decision making. Neuropsychopharmacology, 43(2), 313-324. https://doi.org/10.1038/npp.2017.161

Gharote, M. A., & Ennis, O. P. (2021). Potential Role of Nicotinamide Supplementation in Prevention of Global Covid-19 Transmission. Journal of Clinical Case Reports Online, 1(1), 1008.

Gilhotra, N., & Dhingra, D. (2014). Possible involvement of GABAergic and nitriergic systems for antianxiety-like activity of piperine in unstressed and stressed mice. Pharmacological Reports, 66(5), 885-891. https://doi.org/10.1016/j.pharep.2014.05.008

Goddard, A. W. (2016). Cortical and subcortical gamma-amino acid butyric acid deficits in anxiety and stress disorders: clinical implications. World Journal of Psychiatry, 6(1), 43-53. https://doi.org/10.5498/wjp.v6.i1.43

Harman, D. (1992). Free radical theory of aging: history. Free radicals and aging, 1-10.

Hinz, M., Stein, A., & Uncini, T. (2020). A Pilot Study Differentiating Recurrent Major Depression from Bipolar Disorder Cycling on the Depressive Pole [Retraction]. Neuropsychiatric Disease and Treatment, 16, 3129-3130. https://doi.org/10.2147/NDT.S296320

Jonscher, K. R., Chowanadisai, W., & Rucker, R. B. (2021). Pyrroloquinoline-quinone is more than an antioxidant: a vitamin-like accessory factor important in health and disease prevention. Biomolecules, 11(10), 1441. https://doi.org/10.3390/biom11101441

Kennedy, E., & Niedzwiedz, C. L. (2022). The association of anxiety and stress-related disorders with C-reactive protein (CRP) within UK Biobank. Brain, Behavior, & Immunity-Health, 19, 100410. https://doi.org/10.1016/j.bbih.2021.100410

Korokov K. & Yanovskaja E. (2024). The Principles of Bio-Well Analysis. Amazon.com publishing: Seattle, WA, USA.

Korotkov K.G. (2004) Measuring Energy Fields, Backbone Publishing Company, Fair Lawn, NJ, USA

Kostyuk, N., Cole, P., Meghanathan, N., Isokpehi, R. D., & Cohly, H. H. (2011). Gas discharge visualization: an imaging and modeling tool for medical biometrics. International journal of biomedical imaging, 2011(1), 196460. https://doi.org/10.1155/2011/196460

Kostyuk, N., Meghanathan, N., Isokpehi, R. D., Bell, T., Rajnarayanan, R., Mahecha, O., & Cohly, H. (2010). Biometric evaluation of anxiety in learning English as a second language. International Journal of Computer Science and Network Security, 10(1), 220-229.

Kulkarni, S., O'Farrell, I., Erasi, M., & Kochar, M. S. (1998). Stress and hypertension. WMJ: official publication of the State Medical Society of Wisconsin, 97(11), 34-38.

Kurl, S., Laukkanen, J. A., Rauramaa, R., Lakka, T. A., Sivenius, J., & Salonen, J. T. (2001). Systolic blood pressure response to exercise stress test and risk of stroke. Stroke, 32(9), 2036-2041. https://doi.org/10.1161/hs0901.095395

Lapenna, D. (2023). Glutathione and glutathione-dependent enzymes: from biochemistry to gerontology and successful aging. Ageing research reviews, 102066. https://doi.org/10.1016/j.arr.2023.102066

Lee, S. A., Hong, S. S., Han, X. H., Hwang, J. S., Oh, G. J., Lee, K. S., M.K Lee, B.Y. Hwang & Ro, J. S. (2005). Piperine from the fruits of Piper longum with inhibitory effect on monoamine oxidase and antidepressant-like activity. Chemical and pharmaceutical bulletin, 53(7), 832-835. https://doi.org/10.1248/cpb.53.832

Lee, S. T. (2020). Inflammation, depression, and anxiety disorder: a population-based study examining the association between Interleukin-6 and the experiencing of depressive and anxiety symptoms. Psychiatry research, 285, 112809. https://doi.org/10.1016/j.psychres.2020.112809

Malhotra, B., Kulkarni, G. T., Dhiman, N., Joshi, D. D., Chander, S., Kharkwal, A., Sharma, A.K. & Kharkwal, H. (2021). Recent advances on Berberis aristata emphasizing berberine alkaloid including phytochemistry, pharmacology and drug delivery system. Journal of Herbal Medicine, 27, 100433. https://doi.org/10.1016/j.hermed.2021.100433

Mehmel, M., Jovanovi?, N., & Spitz, U. (2020). Nicotinamide riboside—the current state of research and therapeutic uses. Nutrients, 12(6), 1616. https://doi.org/10.3390/nu12061616

Niemira, B. A. (2012). Cold plasma decontamination of foods. Annual review of food science and technology, 3(1), 125-142.

Nogara, L., Naber, N., Pate, E., Canton, M., Reggiani, C., & Cooke, R. (2016). Piperine’s mitigation of obesity and diabetes can be explained by its up-regulation of the metabolic rate of resting muscle. Proceedings of the National Academy of Sciences, 113(46), 13009-13014. https://doi.org/10.1073/pnas.1607536113

Ogawa, S., Ota, M., Ogura, J., Kato, K., & Kunugi, H. (2018). Effects of L-theanine on anxiety-like behavior, cerebrospinal fluid amino acid profile, and hippocampal activity in Wistar Kyoto rats. Psychopharmacology, 235, 37-45. https://doi.org/10.1007/s00213-017-4743-1

Pan, Y., Cheng, J. H., & Sun, D. W. (2019). Cold plasma?mediated treatments for shelf life extension of fresh produce: A review of recent research developments. Comprehensive Reviews in Food science and Food safety, 18(5), 1312-1326. https://doi.org/10.1111/1541

Park, M. I., & Park, Y. J. (1997). Investigation on the tachyonic neutrino. Modern Physics Letters A, 12(28), 2103-2114.

Patel, P. (2021). A bird's eye view on a therapeutically ‘wonder molecule’: Berberine. Phytomedicine Plus, 1(3), 100070. https://doi.org/10.1016/j.phyplu.2021.100070

Peng, A., Li, J., Xing, J., Yao, Y., Niu, X., & Zhang, K. (2024). The function of nicotinamide phosphoribosyl transferase (NAMPT) and its role in diseases. Frontiers in Molecular Biosciences, 11, 1480617. https://doi.org/10.3389/fmolb.2024.1480617

Rastogi, R., Saxena, M., Chaturvedi, D. K., Gupta, M., Gupta, N., Agrawal, U., Srivastava, Y. & Gau, V. (2021). Measurement of human bioelectricity and pranic energy of different organs: A sensor and CPS-based approach. In Computational Intelligence in Healthcare (pp. 1-33). Cham: Springer International Publishing.

Schnabel, U., Niquet, R., Schlüter, O., Gniffke, H., & Ehlbeck, J. (2015). Decontamination and sensory properties of microbiologically contaminated fresh fruits and vegetables by microwave plasma processed air (PPA). Journal of Food Processing and Preservation, 39(6), 653-662. https://doi.org/10.1111/jfpp.12273

Sumi?ska, S. (2022). Measurement of stress response in laboratory settings-a review of studies using stress induction protocols. Medycyna Pracy, 73(3), 251-270. https://doi.org/10.13075/mp.5893.01109

Tabares, F. L., & Junkar, I. (2021). Cold plasma systems and their application in surface treatments for medicine. Molecules, 26(7), 1903. https://doi.org/10.3390/molecules26071903

Tomei, F., Ricci, S., Gioffrè, P. A., Sacco, C., Antetomaso, L., Pagliara, F., & Tomei, G. (2016). Perceived stress and hepatic parameters. Prevention and research, 5(4), 118-129

Valverde, R. (2016). A quantum biofeedback and neurotechnology cybertherapy system for the support of transpersonal psychotherapy. NeuroQuantology, 14(4),161-178. https://doi.org/10.14704/nq.2016.14.4.963

Valverde, R. (2022). Transpersonal Psychology: Altered States of Consciousness, Biofeedback and Neurotechnology. Bentham Science Publishers.

Valverde, R., Gavrilova, E., Churganov, O., & Korotkov, K. (2023b). Influence of Enhanced Amino Acid Compositions on Human Cognitive Functioning. HSOA Journal of Alternative, Complementary & Integrative Medicine, 9, 377. http://dx.doi.org/10.24966/ACIM-7562/100377

Valverde, R., Gavrilova, E., Churganov, O., Korotkov, K., & Starchenko, M. (2023a). Influence of enhanced Amino Acid Compositions on Human Cognitive Functions after COVID-19. Journal of Alternative, Complementary & Integrative Medicine, 9(6), 370. http://dx.doi.org/10.24966/ACIM-7562/100370

Valverde, R., Korotkov, K., & Swanson, C. (2023c). Measuring Altered States of Consciousness in Transpersonal Psychotherapy with Gas Discharge Visualization Technology: A Systematic Review. Journal of Consciousness Exploration & Research, 14(6,479-504.

Valverde, R., Toleman, M., & Cater-Steel, A. (2011). A method for comparing traditional and component-based models in information systems re-engineering. Information Systems and e-Business Management, 9, 89-107. https://doi.org/10.1007/s10257-010-0139-z

Vetvicka, V., & Vetvickova, J. (2014). Anti-stress action of an orally-given combination of resveratrol, ?-glucan, and vitamin C. Molecules, 19(9), 13724-13734. https://doi.org/10.3390/molecules190913724

Vitetta, L., Anton, B., Cortizo, F., & Sali, A. (2005). Mind?body medicine: Stress and its impact on overall health and longevity. Annals of the New York Academy of Sciences, 1057(1), 492-505. https://doi.org/10.1111/j.1749-6632.2005.tb06153.x

Wang, K., Chen, Q., Wu, N., Li, Y., Zhang, R., Wang, J., & Chen, J. (2019). Berberine ameliorates spatial learning memory impairment and modulates cholinergic anti-inflammatory pathway in diabetic rats. Frontiers in pharmacology, 10, 1003-1016. https://doi.org/10.3389/fphar.2019.01003

Williams, J. L., Everett, J. M., D’Cunha, N. M., Sergi, D., Georgousopoulou, E. N., Keegan, R. J., & Naumovski, N. (2020). The effects of green tea amino acid L-theanine consumption on the ability to manage stress and anxiety levels: a systematic review. Plant foods for human nutrition, 75(1), 12-23. https://doi.org/10.1007/s11130-019-00771-5

Wu, G. (2013). Functional amino acids in nutrition and health. Amino acids, 45, 407-411. https://doi.org/10.1007/s00726-013-1500-6

Xu, Z., Feng, W., Shen, Q., Yu, N., Yu, K., Wang, S., & Guo, Y. (2017). Rhizoma coptidis and berberine as a natural drug to combat aging and aging-related diseases via anti-oxidation and AMPK activation. Aging and disease, 8(6), 760-777. https://doi.org/10.14336/AD.2016.0620

Yang, L., Ye, Q., Zhang, X., Li, K., Liang, X., Wang, M., & Zhang, X. (2021). Pyrroloquinoline quinone extends Caenorhabditis elegans’ longevity through the insulin/IGF1 signaling pathway-mediated activation of autophagy. Food & Function, 12(22), 11319-11330. https://doi.org/10.1039/d1fo02128a

Ye, Q., Apsley, A. T., Etzel, L., Hastings, W. J., Kozlosky, J. T., Walker, C., & Shalev, I. (2023). Telomere length and chronological age across the human lifespan: A systematic review and meta-analysis of 414 study samples including 743,019 individuals. Ageing Research Reviews, 102031. https://doi.org/10.1016/j.arr.2023.102031

Yu, Y., Zhou, L., Yang, Y., & Liu, Y. (2018). Cycloastragenol: An exciting novel candidate for age associated diseases. Experimental and therapeutic medicine, 16(3), 2175-2182. https://doi.org/10.3892/etm.2018.6501

Published

2025-02-28

How to Cite

Valverde, R., Gavrilova, E., O.A., C., & Korotkov, K. (2025). Influence of an Amino Acid Composition enhanced with Cold Plasma Radiation on Psychological Stress: A Blood Test, Gas Discharge Visualisation and Biofeedback Approach. International Journal of Studies in Psychology, 5(1), 28-34. https://doi.org/10.38140/ijspsy.v5i1.1616